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Copyright=20 © Frontiers in Bioscience,
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[Frontiers in = Bioscience=20 14, 4008-4014, January 1, 2009]

Effects of transgenic Bt+CpTI cotton on the = growth and=20 reproduction of earthworm Eisenia foetida

Biao=20 Liu1, Jinjie Cui2, Jun Meng1, = Wenjun=20 Hu3, Junyu Luo2, Yangping Zheng1=20

1State Key Biosafety Laboratory, Nanjing = Institute=20 of Environmental Sciences(NIES), Ministry of Environmental = Protection of=20 China, Nanjing, Jiangsu 210042, China; 2 Cotton = Research=20 Institute, Chinese Academy of Agricultural Sciences, Anyang, Henan = 455000,=20 China, 3 School of Life Science and Technology, = China=20 Pharmaceutical University, Nanjing, Jiangsu 210009, China

TABLE OF CONTENTS

1. Abstract=20
2. Introduction=20
3. Materials and methods=20
4. Results=20
4.1. Effects of acute toxicity of transgenic Bt+CpTI cotton = on E.=20 foetida=20
4.2. Effects of transgenic Bt+CpTI cotton on SOD Activity of = E.=20 foetida=20
4.3. Effects of transgenic Bt+CpTI cotton on the growth of = E.=20 foetida=20
4.4. Effects of transgenic Bt+CpTI cotton on the = reproduction of E.=20 foetida=20
5. Discussion=20
6. Acknowledgments=20
7. References

1. ABSTRACT

With the expansion of the planted area of = transgenic=20 Bt+CpTI cotton, the effects of this crop on non-target organisms = in soil,=20 including earthworms, are becoming the most important aspect of = their=20 ecological risk assessment. Laboratory toxicity studies were = conducted to=20 determine the effects of transgenic Bt+CpTI cotton leaves, = containing high=20 concentrations of the Bt toxin and cowpea trypsin inhibitor, on = the=20 earthworm Eisenia foetida. In comparison with the = non-transgenic=20 cotton line Zhong23, transgenic Bt+CpTI cotton Zhong41 had no = significant=20 acute toxicity on E. foetida. Moreover, the leaves of = transgenic=20 Bt+CpTI cotton were more suitable than the non-transgenic cotton = Zhong23=20 for E. foetida growth and reproduction (time of = reproduction, the=20 number of cocoons and newly incubated offspring).=20

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